Mutation screening techniques and sequencing (including pyrosequencing and Next generation sequencing).Basic techniques such as amplification by Polymerase Chain Reaction (PCR), including primer design DNA ligation and nucleic acids hybridization techniques, necessary to understand more sofisticated and specific protocols.Then, the most up to date techniques applied to molecular diagnostics as well as their main applications will be described: data to be included in the clinical report regarding molecular diagnoses.development of molecular tests, including their requirements in terms of specificity, sensitivity and other clinical parameters.
procedures to set up an accredited and certified laboratory of Molecular Diagnostics,.Additional activities may be planned each year, such as visits at Molecular Diagnostics accreditated centers or seminars held by professionals employed in relevant Molecular Diagnostics structuresĬlass lectures will deal with the following topics: Basic knowledge of microbiology and immunology.Ĭlass lectures (32 hours). Knowledge of genetics and molecular biology (particularly PCR). ability to resume and present scientific information development of effective communication skills by practicing, reading, writing and speaking clearly ability to identify and express relevant information ability to apply an analytical, problem-solving, approach ability to apply the knowledge acquired in the field of Molecular Diagnostics to make decisions on clinical tests to be applied to particular diagnostic queries. ability to acquire independence in searching data, information, methodologies in order to set up and validate new diagnostic protocols ability to evaluate papers dealing with methods or clinical applications and to interpret the correct execution and interpretation of a molecular test ability to read, understand and criticize a scientific text (in English) knowledge of up to date methods in Molecular Biology and Molecular Genetics, including their theoretical bases The course will also deal with the requirements to set up a molecular diagnostics laboratory and obtain certification and accreditation and to choose the test menu to offer. The course will describe the techniques commonly used in diagnostics and molecular pathology laboratories and the underlying principles and applications, advantages and limitations of each technique. The Molecular Diagnostics Laboratory complements these tests by performing in situ hybridization and quantitative PCR to assay for Epstein-Barr virus and supports the 16S ribosomal RNA DNA sequencing test to identify bacteria.The main objective of the course in Molecular Diagnostic Techniques is to make the student familiar to the procedures used in a Laboratory of Molecular Diagnostics. The Clinical Virology Laboratory tests for multiple viral pathogens, whereas the Clinical Microbiology Laboratory tests for M. Other department laboratories perform molecular testing for microorganisms. We also perform several additional genetic tests including hemochromatosis and cystic fibrosis screens for adults and in conjunction with the prenatal laboratory for newborns.Ī listing of our testing can be found here. Hematopathology is a main focus of our lab with testing performed in genetic analysis of hematologic malignancies for diagnosis and therapeutic decision-making, coagulation genetics, and evaluation of stem-cell transplant patients. The three broad areas of testing we provide are: We offer a wide array of tests at the forefront of molecular diagnostics and precision medicine. The Molecular Diagnostics Laboratory is responsible for the development and performance of molecular diagnostic tests for nucleic acid targets found in a variety of settings in medicine.
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